Premier Analytical Services

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GM Determination

Genetically Modified Organisms detection and Quantification Services

The presence of Genetically Modified Organisms (GMOs) in foods remains a major issue for consumers. European Union (EU) regulations have been designed to ensure that the consumers’ right to choose what they eat is facilitated by full and frank ingredient disclosure. In order to achieve this all ingredients that contain or consist of GMOs, or contain ingredients produced from GMOs must be labelled as such.

Harmonised EU systems regarding documentation make it easier to trace identity preserved (IP), non-GM, ingredients throughout the supply chain. Legislation also permits a certain amount of adventitious contamination of I.P. ingredients:

  • A threshold of 0.9% of the ingredient applies for the accidental presence of approved GM material below which labelling of food or feed is not required.
  • However there is no threshold for the presence of GM material that has not been approved for use in the EU.

Routine analysis of IP ingredients and products, for due diligence demonstration of GMO threshold compliance, remains an important part of any HACCP control. Now more than ever before this GMO analysis must feature accurate and reliable quantification.

Premier Analytical Services’s GMO Detection and Quantification Service assures confidence in results you can trust with the first method accredited by UKAS, to the ISO 17025 standard, for the detection and QUANTIFICATION of GM materials in both PROCESSED FOODS AND INGREDIENTS.

Our dedicated team utilise four separate laboratories to avoid sample cross contamination. They employ the real time polymerase chain reaction (PCR) technique using TaqMan chemistry with custom designed primers and probes.

All Part of the Service

Advice Based on Experience

Premier Analytical Services was the first UK laboratory to offer a UKAS accredited quantitative GMO testing service in the UK utilising analysis based on real-time PCR. More than 20 years of experience in molecular diagnostic techniques has meant that our scientists have been at the forefront of the detection and quantification of GMOs, providing expert advice to UK and European governments. This advice is readily available to all our customers in terms of recommendations regarding sampling, sampling frequency and testing regimes.

Accuracy and Precision

  • An extensive DNA extraction process is followed by advanced purification, in order to remove inhibitors of the PCR reaction
  • Certified Reference Materials are used in each analysis to enable accurate quantification
  • Appropriate positive and negative controls are used with each analysis
  • Multiple primers and probes are employed. These have been designed for specific and sensitive detection of a wide range of GMOs
  • Analysis is carried out in triplicate; we do however offer the ability to run a greater number of replicates if required.

Sensitivity
The limit of detection for both maize and soya in processed and raw materials can be calculated for each sample analysed. These are typically 0.01% for processed foods and 0.001% for raw materials.

Interpretation of Results
Premier Analytical Services’s GMO Detection and Quantification Service can provide a full explanation and interpretation of all results thus enabling you to take the appropriate actions.

Quality Assurance
Exemplary performance in collaborative proficiency testing schemes further endorses the accuracy of our work, which is performed to the ISO 17025 standard as monitored by UKAS.

Three-Phased Approach
The possible combinations of GMOs that could be present in food materials are becoming increasingly complex as more GMOs receive EU approval. Premier Analytical Services has developed a three-phased approach to GMO detection and quantification in order to minimise costs. Details are tabulated below.

Premier Analytical Services’s GMO detection and Quantification Service:
Three Phased Approach

Phase Analysis Targets UKAS Accredited
Phase 1- General Screen Screen to detect most common component of GMOs coupled with the detection of indigenous marker genes CaMV35S promoter sequence plus Soya lectin and Maize zein indigenous marker genes YES
Phase 2 - Identification Samples positive for CaMV35S and the soya marker gene Establish the presence of Roundup Ready Soya YES
  Samples positive for CaMV35S and the maize marker gene Establish the presence of:  
    Bt176, Mon 810, Bt11 YES
    T25, NK 603 and Starlink (CBH 351) NO
Phase 3 - Quantification Quantification of GM Soya Roundup Ready Soya YES
  Quantification of GM Maize Bt 176 YES
    Mon 810 NO
    Bt11 NO

 

FIRST FOR FOOD ANALYSIS
FIRST FOR SCIENTIFIC INTEGRITY